Research
Development and Evaluation of a Novel Herbal Emulgel for Potential Anti-Inflammatory and Antioxidant Activities in Vitro
Authors:
T. L. I. Srilal,
University of Ruhuna, LK
About T. L. I.
Department of Pharmacy, Faculty of Allied Health Sciences
S. K. Hettihewa
University of Ruhuna, LK
About S. K.
Department of Pharmacy, Faculty of Allied Health Sciences
Abstract
Emulgel is a novel topical drug delivery system which can overcome the major limitation of gel; limited delivery of hydrophobic drugs. Leea indica (Burm.f.) Merr. (Burulla) is a medicinal plant used in Sri Lankan Ayurvedic medicine for many therapeutic purposes. The present study was aimed to develop a novel herbal emulgel using Leea indica leaf extract and evaluate in vitro anti-inflammatory and antioxidant activities. Different formulations (Fg1-Fg5) were prepared by incorporating freeze dried powder of 70% aqueous acetone leaf extract. All formulations (Fg1-Fg5) were tested for their physicochemical stability parameters such as pH, appearance, odor, homogeneity, spread ability, phase separation and washability for 60 days and they were subjected for in vitro anti-inflammatory activity by Human Red Blood Cell membrane stabilization assay and in vitro antioxidant activity by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay respectively. Two commercially available gels were used as the positive control. Among the formulated herbal emulgels, Fg5 showed the significantly high values of in vitro anti-inflammatory and antioxidant activities compared to the tested positive controls. At the concentration of 12.5 mg/mL, Fg5 showed percentage inhibition of 93.73 ± 0.90% of heat induced hemolysis of erythrocytes and in vitro antioxidant activity of Fg5 was 3.39 ± 0.01 mmol Trolox per 100 g of emulgel. All formulated emulgels were found to be semi-solid, homogenous, and washable with water, having no phase separation and pH of the formulations were reported in the range of 6 to 7.There was no remarkable variation ofstability parameters tested during 60 days. It isconcluded that the formulated herbal emulgelshave promising anti-inflammatory andantioxidant activities and formulation Fg5 canbe recommended for further investigationsusing in vivo models.
How to Cite:
Srilal TLI, Hettihewa SK. Development and Evaluation of a Novel Herbal Emulgel for Potential Anti-Inflammatory and Antioxidant Activities in Vitro. CINEC Academic Journal. 2021;5(1):46–53. DOI: http://doi.org/10.4038/caj.v5i1.73
Published on
05 Jan 2021.
Peer Reviewed
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